master mix iqtm sybr green supermix  (Bio-Rad)

Journal: Nutrition & Metabolism

Article Title: Inflammation rapidly modulates the expression of ALDH1A1 (RALDH1) and vimentin in the liver and hepatic macrophages of rats in vivo

doi: 10.1186/1743-7075-11-54

Figure Lengend Snippet: ALDH1A1 mRNA relative expression levels in 8-wk old rats fed chow diet (A and B) and in VAA and VAD rats (C). Total RNA was extracted from the liver samples of individual rats and quantified by real time PCR with SYBR Green for ALDH1A1 and 18S ribosomal RNA (rRNA) (A and C) . Upon completion, the PCR products from individual samples in Figure 1A were pooled in each group and subjected to ethidium bromide agarose gel electrophoresis, with DNA molecular weight markers (MWM) (B) . Values in A and C were individually normalized to 18S RNA and are expressed as the mean ± SEM of n = 4-6/group. Groups not sharing a common letter were significantly different, P <0.05 (a > b > c).

Article Snippet: Total RNA was extracted from liver tissue using methods previously described [ ] using TRIzol reagent (Life Technologies, Carlsbad, CA). cDNA was synthesized using M-MLV reverse transcriptase (Promega Co., Madison, WI) and qRT-PCR analysis was performed using 2× iQTM SYBR® Green supermix PCR Master Mix (BioRad, Hercules, CA).

Techniques: Expressing, Real-time Polymerase Chain Reaction, SYBR Green Assay, Agarose Gel Electrophoresis, Molecular Weight